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After washing with PBS, cells were pelleted and incubated with Annexin V-FITC/Propidium Iodide solutions (eBioscience Annexin V Apoptosis Detection Kit, Invitrogen by Thermo Scientific, Waltham, MA, USA), according to the manufacturer instructions

Though the procedure is non-invasive and typically safe, some people may experience redness or irritation for a short period, particularly those with sensitive skin

Other stroke models include: intraluminal suture, craniotomy model, photothrombosis model, endothelin-1 model, embolic stroke model 130

A single session can suit someone with a very small amount of fat who wants a subtle change, but for a clear, even result a short course almost always works better than one visit

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