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In this study, Clementi et al observed higher eryptosis levels in patients with advanced CKD (stage G4 and stage G5), compared with early stages of chronic renal damage (stage G1, G2, and G3) [260]

Hepatology 66 , 602615 (2017)

For fluorescence measurements, 20 l samples were removed at distinct time points and mixed with 180 l of a 55.6 M solution of Thioflavin T (ThT) (SigmaAldrich) in 0.5 PBS and analyzed in a FluoroMax-3 spectrofluorometer (HORIBA Jobin Yvon, Bensheim, Germany) using an excitation wavelength of 450 nm and an emission wavelength of 482 nm

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For the thigh specifically, injection volumes above 0.5mL benefit from an even slower injection speed