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Therefore, the effects of GH replacement on muscle and bone in GH-deficient individuals are significant and beneficial, although the longer-term effects of GH replacement in terms of reducing the number of fractures and prevention of frailty in old age are not yet established

doi: 10.3389/fgene.2022.1021423 Received 03 October 2022 Accepted 29 November 2022 Published 04 January 2023 Volume 13 - 2022 Edited by Lindsay Fernandez-Rhodes, The Pennsylvania State University (PSU), United States Reviewed by Shifu Li, Central South University, China Reza Jabal, Albert Einstein College of Medicine, United States Updates Copyright 2023 Zhao, Li, Dang, Li, Fan, Hao, Song, Lu, Lu, Jian, Wang, Wang, Wu and Zhang

For single-session in-vitro work, sterile saline is acceptable

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