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The absorbance change of the system is detected at a wavelength of 540 nm to calculate the enzyme activity.Definition of glucoamylase activity: Under the conditions of 40 C and pH 4.6, the amount of glucose produced by 1 h of crude enzyme solution is defined as one enzyme activity unit (U), where 1 unit (U) is the amount of glucose (1 mg) generated by the crude enzyme solution within 1 h

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