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Two slides were prepared for each subject and a blind scorer examined 50 randomly selected cells from each slide (100 cells/subject) using a 200 magnification in a Axioplan2 imaging epifluorescence microscope (Carl Zeiss Microscopy, Gttingen, Germany) with an image analysis system (Comet Imager 2.2 Software, MetaSystems, Altlussheim, Germany)