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The supernatant was also saved (tube 2) and the loosened epithelium was then manually scraped off into stock solution with 10% FBS (tube 3), and all cells were collected by centrifugation for 10 min at 225 g , 4 C (tubes 1, 2, and 3)

"Almost everyone in the school has a Juul (e-cigarette)," said John, a 16-year-old junior at Bearden who would agree to be identified only by his first name

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