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362 NAD + deficiency correlates with disturbed circadian clocks during aging Besides mitigating the oxidative damage, NAD + can extend lifespan by driving the circadian rhythms

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To study gene expression, total RNA was extracted from half-colon samples using phenol-chloroform methods and purified with lithium chloride precipitation, which is particularly important to remove residual DSS polysaccharides that can interfere with downstream reactions