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The injection itself causes microscopic bleeding into surrounding tissue, and your body gradually reabsorbs this blood over 1-3 weeks, creating the characteristic purple-to-yellow color progression

The concentration of Mouse MIP1a in the samples is then determined by comparing the OD of the samples to the standard curve

A., Khalil, E

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Notably, the presence of AMF had a more pronounced effect when paired with higher ECT concentrations, showing an increase of approximately 24.43% with 0.25 mM ECT and 19.44% with 0.50 mM ECT, both compared to the corresponding treatments without AMF (Fig