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Indeed, high NAD + levels and NAD + /NADH ratios are associated with increased energy production in humans, improved mitochondrial membrane potential, and decreased mitochondrial mass through mitophagy in vitro, suggesting improved efficiency of mitochondria 9497

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The membranes were then rinsed with PBS three times (5 min each), followed by an incubation in blocking solution (30% Roti Block (Carl Roth, Karlsruhe, Germany)) in Tris-Buffered Saline with 0.1% Tween 20 (TBST