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A 2016 study published in Nature Scientific Reports showed that GHK-Cu had no cytotoxicity to human keratinocytes at concentrations up to 5,800 micromolar over 72 hours and did not induce inflammatory biomarkers

This stability is attributed in part to the presence of proline residues, which help protect the peptide from enzymatic breakdown and support its potential as a research chemical in studies involving the gastrointestinal tract and other tissues

The study aimed to elucidate the neurochemical changes associated with different treatment regimens

pylori stool antigen test were included in a 4-week study and randomized to one of three groups: standard triple therapy (twice daily 20 mg omeprazole, 500 mg clarithromycin, 1,000 mg amoxicillin for 14 days), broccoli sprout powder (6 g/day), or a combination of broccoli sprout powder and standard triple therapy

Briefly, GSH assay buffer, GSH reductase, 5,5-dithio-bis 2-nitrobenzoic acid solution and supernatant sample were mixed together and incubated at 25C for 5 minutes, then NADPH was added into this system to trigger the reaction