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The proportion of bacterial cells that remained attached to the HT29-MTX monolayer was determined to reflect the extent of specific host-microbe interaction

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Guangxi Health Commission Key Laboratory of Applied Fundamental Research of Zhuang Medicine (Guangxi University of Chinese Medicine) (Gui Wei Ke Jiao Fa (2020) No.17)

Cells were washed once in serum-free RPMI medium and resuspended in serum-free RPMI medium

To test this, splenic CD4 + T cells were isolated from wildtype C57BL/6J mice and activated with plate-bound antibodies to CD3 and CD28 in serum free media supplemented with either Insulin Free Media Supplement or bovine serum albumin (BSA) to prevent any confounding effect of growth factors or hormones found in serum