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Description

Plasmid constructs FLAG-UBR5 (#37188), HA-GSK3 (#14753), HA-GSK3-S9A (#14754), and HA-GSK3-K85A expression plasmids were from Addgene (USA)

Freshly prepared AX in PBS was incubated in the absence or presence of purified HSA purified from serum for 16 h at 37C (final concentrations of HSA and AX in incubation mixture were 3 mg/ml and 2.5 mg/ml, respectively)