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doi: 10.3389/fneur.2020.590059 47 BredesenD

Binding of BRD4 ET to ASXLs is conserved ASXL1 and homologous ASXL2 and ASXL3 were identified as mutually exclusive components of the PR-DUB complex 1,29 , and all contain similar but not identical ET-binding sequences (IRIQL in ASXL1, LKIPV in ASXL2 and LKIQL in ASXL3) (Supplementary Fig

Serum (thawed on ice) metabolites were extracted by adding 200 L of methanol at 80 C to 50 L of the serum sample, followed by vortexing for 5 min, incubation on ice for 10 min, and centrifugation at 4 C and 12,000 g for 15 min