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5) Inflammation High TMAO levels were associated with higher levels of TNF-alpha, an inflammatory cytokine, but not with IL-6 or CRP, in 271 adults [24]

The study was conducted in accordance with the standards of the Declaration of Helsinki and was approved by the ethics committee of the Freiburger Ethik-Kommission International (FEKI, feki Code: 016/1224)

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After RNase A digest, 12 ng of the isolated and cleaned DNA was used for library preparation in a 2-step PCR