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Clinical Background (Summary and Explanation of the Test) Glucose-6-phosphate dehydrogenase (G-6-PD) is a cytoplasmic enzyme that is distributed in all cells

Allard, D

Briefly, the assay involves the enzymatic conversion of glucose to glucose-6-phosphate coupled with glucose-6-phosphate dehydrogenase, and the NADH thus formed reduces a colorless probe to a colorimetric product, which is then read at 450 nm