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3, this protocol was found to be general and robust, with remarkable functional group tolerances

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The sections were stained with hematoxylin and eosin and images were taken with a Nikon ECLIPSE Ni Upright Microscope (Nikon, Melville, NY) and ONL thickness at six locations relative to the optic nerve were measured: 25% (S3), 50% (S2), and 75% (S1) of the distance between the superior pole and the optic nerve and 25% (I3), 50% (I2), and 75% (I1) of the distance between the inferior pole and the optic nerve

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If this is the case, make sure you are still sticking within the recommended MIC b12 injection dosage range and speaking with your medical provider regularly