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Methylation sequencing NCI-H125 pellet collection was performed 48 h after NKX21 knockdown after culturing the cells for 4 h in serineglycine-glucose-free RPMI-1640 (Teknova) supplemented with 2 mg/ml glucose (Sigma-Aldrich) and with 10% dialyzed serum (Life Technologies, A3382001)

Add the solvent slowly down the vial wall, swirl gently never shake

Assay performance and Validation of NADMED Assay Against Mass-Spectrometry NADMED assays demonstrated high precision with intra-assay coefficients of variation (CV%) ranging from 2.28 to 9.02, and inter-assay precision from 3.93 to 9.37

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