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The eluates were recovered and neutralized to pH: 6.5 using 0.1 mL of buffering solution [tris(hydroxymethyl)aminomethane solution at pH: 8.5] and stored at 80C until antibody analysis

J Nutr 66(2):291303 Mu Y, Xiang N, Zuo X, Yu X, Chen J (2021) Effects of total glucosides of Paeonia lactiflora on intestinal mucosal barrier and intestinal flora in rats with autoimmune thyroiditis

Published data suggests up to 2.6% incidence of B12 deficiency within a year of treatment, and this figure climbs in patients who have used these medications long term or combined them with very low calorie diets (3)

This is called inflammation

In both BacA and wild-type groups the survival rate of MAP in macrophages alone was significantly higher than macrophages incubated with PBMCs, regardless of PBMCs being primed ( Figure 8B ), suggesting that effector T cells contribute to the killing of MAP