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First, autophagy plays a protective role by inhibiting oxidative damage and apoptosis in CuSO 4 -induced testicular injury and spermatogenesis disruption, and second, autophagy plays an adverse role by promoting ferroptosis, thus aggravating the toxicity caused by CuSO 4 [100]

The zinc coordination is more static, and zinc is consistently bound to three hisidine and one aspartate side chain (see Figure below)

The Pyruvate Dehydrogenase complex oxidizes pyruvate produced by glycolysis to acetyl-CoA and is a major control point of TCA cycle activity for carbohydrate metabolism (Gray et al., 2014)

Then these are sealed by sealing machine in an appropriate manner