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J.Gil-IzquierdoA.MedinaS.OsorioE.Alvarez-QuinteroR.ZuluagaN.et al

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Organ suspensions were subjected to serial 3-, 5- or 6-fold dilutions (depending on the state of disease progression) in modified Schneides Drosophila medium (Leitherer et al., 2017), with 12 replicates per dilution step

Without adequate methylation processes, the individual cannot adapt effectively and will suffer the deleterious effects of accelerated aging (1)

A similar study examining the impact of CA on the epigenome in a general population found that tobacco consumption was an important confounding when examining the signature of CA (Marzi et al., Reference Marzi, Sugden, Arseneault, Belsky, Burrage, Corcoran and Caspi2018)