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Samples were mixed for 5 min and centrifuged again at 2000 rpm for an additional 5 min

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Melanocortin and Metabolic Peptides: A Different Research Category Entirely Peptides like Melanotan II (melanocortin receptor agonist) and GLP-1 receptor agonists like Semaglutide and Tirzepatide act through completely different receptor systems melanocortin receptors and GLP-1 receptors respectively with no mechanistic overlap with BPC-157

These results confirmed that by upregulating the level of Bax/Bcl-2, the H 2 O 2 could promote the mitochondria to release cyt-C to the cytoplasm and activate caspase-9 and caspase-3 which are the downstream apoptotic proteins of mitochondrial pathway