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Flavonoids can prevent the formation of damaging radicals, such as hydroxyl radicals formed via the Fenton reaction by the chelation of redox-active metals (copper, iron, manganese) (Simunkova et al

Amicon Ultra, Millipore), and run on a Superdex 200 (GE healthcare) in buffer containing 25 mM HEPES pH 7.5, 200 mM NaCl, and 1mM DTT

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A diverse spectrum of bioactive dietary ingredients, including curcumin, epigallocatechin-3-gallate (EGCG), genistein, quercetin, resveratrol, and sulforaphane, has gained significant interest for their ability to modulate gene expression and chromatin structure via epigenetic mechanisms