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The isolated monocytes were plated in modified delberg's culture medium (IMDM) ex vivo (ThermoFisher) containing 10% fetal bovine serum in 24-well plates overnight and non-adherent cells were washed off after 24 hours

and (3) concentrations of MCFAs (Saerens et al

Any temperature excursion above 8C for more than 2 hours causes irreversible peptide denaturation

As the leading liver disease worldwide, MASLD became a coveted pharmaceutical target and yielded unprecedented insights into the relevance of several signaling cascades

Thus, elucidation of the molecular mechanisms of kidney disease progression is urgently needed