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Calibration curves for HepG2 and HepG2 ATP7B-KO cells were generated according to manufacturers instructions by plating 40,000, 20,000, 10,000, 5,000, 2,500, 1,250, 652, 312, 156 and 0 cells in 100 l Medium without CuCl 2 and CuSO 4 in a 96-well-plate

GHK-Cu , , : ~200 / 20 ~80 / 60+

Laboratory studies have demonstrated biological activity at concentrations as low as 0.01 nanomolar, showing measurable effects on fibroblast activity and collagen production even at these minute levels

Using an insulin syringe, draw the calculated volume of bacteriostatic water (typically 2 mL)