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As is well-known, The gut microbiota is responsible for the production of various metabolites, including SCFAs and bile acids, which play critical roles in maintaining gut health and regulating immune responses ( Alterations in the composition of gut microbiota can disrupt the production of these metabolites, leading to an increased risk of metabolic disorders and liver disease ( Detection techniques and factors influencing microbiota research results The inconsistency of gut microbiota research results in CLDs is partly attributed to differences in detection techniques and research factors

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The level of NO was detected, using the Griess-Illosvoy reagent, based on the previous method 15,83