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Naturally released by the human gut and vital in appetite regulation and weight management, research has revealed that supplementation in obese patients can help reduce the latter's BW by 20% or more

The mobile phase consisted of A: 60:40 acetonitrile:water (v/v) and B: 90:10 isoproanol:acetonitrile (v/v), both with 10 mM ammonium formate, 0.1% formic acid and 0.1 M reserpine (lock mass)

The reduced toxicity was attributed to the presence of abundant oxygen-containing functional groups and a smoother morphology due to microbial capping agents, which improved biocompatibility

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ICP-MS measurements To quantify the possible presence and concentration of metal ions in GLT25D1/COLGALT1 recombinant preparations, 900 L of a sample containing 4 mg mL 1 GLT25D1/COLGALT1 (purified without usage of divalent ions) were treated with 300 L 65% ultrapure HNO 3 and 200 L 30% w/w H 2 O 2 , made up to 5 mL with ultrapure water and analyzed on a single quadrupole inductively coupled plasma mass spectrometer (SQ-ICP-MS, iCAP RQ Thermo Fisher Scientific), equipped with a quartz cyclonic chamber cooled at 3 C, a MicroMist nebulizer 179 (400 L min 1 ), a quartz torch, a Ni sampler, skimmer cones, and a QCell pressurized with helium (3 V, KED mode)